Abstract

BIOCHEMICAL ESTIMATION OF PRIMARY METABOLITES AND ANTIOXIDANT ACTIVITY OF MADHUCA LONGIFOLIA L.

Dileep Kumar*, Priyanka Kumari and R.A. Shrama

Abstract

The present study was aimed for isolation and quantification of Primary Metabolites from selected plant species. Antioxidant activity of plants was also carried out by using FRAP, Catalase and Peroxidase assay. In Madhuca longifolia L. the maximum amount of primary metabolites was protein (150 mg/gdwt) and the minimum was TSS (4 mg/gdwt ) in leaves. Thus, the leaves of Madhuca longifolia L. were the rich source of protein. In the case of Madhuca longifolia L. leaves was found to be better antioxidant when observed by Peroxidase and Catalase assay. When different samples were analyzed by FRAP assay it was observed that seeds had better antioxidant activity as compared to leaves. The present results showed potential of this medicinal plant which can be used as herbal drug as therapeutic ventures in future aspects.

Keywords: Primary Metabolites; Antioxidant activity; FRAP assay; Catalase assay; Peroxidase assay; Madhuca longifolia L.