Abstract

HPTLC FINGERPRINTING AND QUANTIFICATION OF SHATAVARIN IV IN EXTRACT AND POLYHERBAL FORMULATIONS

Mayur R. Bhurat*, Rupal S. Sanghavi, Sanjay A. Nagdev and Dr. P. R. Patil

Abstract

A new, simple, sensitive, precise and robust high-performance thin layer chromatographic (HPTLC) method was developed for the estimation of Shatavarin IV in herbal extracts and polyherbal formulation. Analysis of Shatavarin IV was performed on TLC aluminium pre-coated plates with silica gel 60F-254 as stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of Chloroform : Methanol, (7 : 3 v/v) for Shatavarin IV at room temperature (25±2oC). After development the plate was derivatized with Anisaldehyde H2SO4. Camag TLC scanner III was used for spectrodensitometric scanning and analysis of plate in absorbance mode at 560 nm for Shatavarin IV. The system was found to give compact spots for Shatavarin IV (Rf 0.46). The data for calibration plots showed good linear relationship with r2 = 0.9993 in the concentration range of 100–600 ng for Shatavarin IV. According to international conference on harmonization (ICH) guidelines the present method was validated for precision, repeatability and recovery. The limits of detection and quantification were determined. Statistical analysis of the data showed that the method is reproducible and selective for estimation of Shatavarin IV.

Keywords: High-performance thin layer chromatography; Method validation; Shatavarin IV; Polyherbal formulations.