Abstract

PRODUCTION AND OPTIMIZATION OF CATECHOL 1, 2- DIOXYGENASE FROM OIL CONTAMINATED SOIL ISOLATES OF BACILLUS SUBTILIS.

N. Suganthi* and S. Deepika

Abstract

This study aimed to evaluate the media conditions for enzyme activity of catechol 1, 2-dioxygenase (C1, 2O) produced by Bacillus subtilis in cell-free extracts. The optimum conditions of pH, temperature, Nitrogen and Carbon sources were determined. Peak activity of C1, 2O occurred at pH 7.0 and 37°C. Among the five nitrogen and carbon sources, highest activity was observed from yeast extract and glucose respectively. After optimization process, enzyme was purified with different method. The enzyme value was improved by gel filtration chromatography than other methods. Furthermore, the enzyme was subjected to molecular characterization with SDS PAGE analysis. The SDS-PAGE analysis reveals that the enzyme has a molecular weight of 30 k Dalton.

Keywords: Biodegradation, Catechol 1, 2-dioxygenase, Catechol, Bacillus subtilis, SDS-PAGE.