Abstract

PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTICANCER ACTIVITY OF TRIDAX PROCUMBENS LINN

Sujitha R. and Dr. R. Sharmila*

Abstract

Objective: To evaluate the phytochemical analysis and invitro anticancer activity of Tridax procumbens linn. To investigate the anticancer potential of ethanolic leaf extract of Tridax procumbens by 3-(4,5-dimethyl-thiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, against MCF 7 (breast cancer cell line). Materials and Methods: The ethanolic extract of T.procumbens leaves was subjected to phytochemical analysis and antibacterial activity was done by disk diffusion method against few bacterial isolates followed by antioxidant studies. The docking studies were performed to check the anticancer potential by insilico method, finally the in vitro anticancer potential of T.procumbens was tested against MCF 7 cell lines through MTT assay. Results: The phytochemical constituents such as phenols, tannins, terpenoids, proteins, carbohydrates were present in our plant. The radical scavenging activity of the leaf extract was maximum at 250μl. The ethanolic leaf extract was found to be more effective against Streptococcus sp with the zone of inhibition of 22mm. The ethanolic extract of T.procumbens has the capability to reduce the size of the formazan crystals at the concentration of 100μg/ml. Conclusion: The present study concluded that the ethanolic leaf extract of T.procumbens has the anticancer potential against MCF cell lines. Further research is needed to isolate and characterize the specific anticancer compound from the plant extract and in vivo studies.

Keywords: T.procumbens, phytochemical screening, antibacterial activity, antioxidant activity, insilico docking, anticancer activity.