Abstract

ANTIBACTERIAL ACTIVITY AND GCMS ANALYSIS OF THE EXTRACT OF LEAVES OF RHIZOPHORA APICULATA (A MANGROVE PLANT)

C. Caleb Johnson Paranjothi and S. R. Murali*

Abstract

The antibacterial screening of hexane, chloroform and alcohol extracts of leaves of Rhizophora apiculata was carried out against pathogenic bacteria viz., Proteus mirabilis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia Coli, Enterococcus faecalis and Proteus Sp. by disc-assay method. The hexane and chloroform extracts were found to be active against four and five pathogens, respectively. Chromatographic purification of active extracts improved the activity profiles. The GCMS analysis of extracts analysis revealed the presence of triterpenes and hydrocarbons as major constituents. The fatty acid composition of the leaf is also studied by FAlv1E analysis. It contains palmitic acid as a major constituent (54.65%). Two important poly unsaturated fatty acids i.e., Linoleic acid (1.54%) and 9,11-0ctadecadienoic acid (3.25%) are also present.

Keywords: Antibacterial activity, Rhizophora apiculata, FA1v1E, hydrocarbons, mangrove plants, triterpenes.