Abstract

PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTI-CANCER ACTIVITY OF FRUIT EXTRACT OF THESPESIA POPULNEA AGAINST HELA AND K562 CANCER CELL LINES

Konda Jeevitha*, Heera Battu, Dharmasoth Ramadevi and Ganga Rao Battu

Abstract

Aim: The purpose of the study was to determine the phyto-chemical constituents and anti-cancer activities of hydro-alcoholic fruit extract of Thespesia populnea on K562 (leukaemia cancer) and HeLa(cervical cancer) cell lines. Methods: Fruit sample of Thespesia populnea was subjected to extraction method with increasing polarity from mintroleum, Ethyl acetate, methanol and hydro-alcohol solvents. The high percentage extractive was found in Hydro-alcohol with soxhlet extraction. Phytochemical constituents of the plant, Thespesia populnea were calculated using different solvents based on polarity (chloroform, ethyl acetate, hydro-alcohol(70:30)(methanol:water) and water). Qualitative analysis of extract was done revealing the presence of flavonoids, phenols, alkaloids, sterols, tannins and reducing sugars. The extract was tested for cytotoxic activities against cancer cell lines, HeLa(cervical cancer) and K562(leukaemia). The hydro-alcoholic extract of fruits is found to be cytotoxic to the cell lines of K562 and HeLa invitro with IC50 values 2886 and 2284 respectively.

Keywords: Anticancer, Phyto-chemical, Thespesia populnea, K562 and HELA.