Abstract

EFFECT OF METHANOLIC EXTRACT OF NERIUM OLEANDER ON SKELETAL MUSCLE OF MICE

Hemantkumar Bhosale*, Mahesh Rode, Vishal Babar, Prajwala Khapale, Pooja Bansude and Jyoti Talekar

Abstract

Objective: The aim of the present investigation was to prepare Methanolic extract leaves of Nerium Oleander & evaluate muscle relaxant activity of Methanolic extract using Rota rod test. Rota Rod Method: The test is used to evaluate the activity of drug interfering with motor coordination. In 1956, Dunham and Miya suggested that the skeletal muscle relaxation introduced by a test compound could be evaluated by testing the ability of mice or rats to remain on revolving rod. This forced motor activity has subsequently been use by investigator. The dose which impairs the ability of 50% of the mice to remain on the revolving rod is considered the endpoint. Results: Methanolic extract of leaves of Nerium Oleander at dose of 25mg/kg shows Skeletal Muscle Relaxant Activity was indicated by decrease in fall of time from Rotating rod in mice as compare with control group of animal. But at 50mg/kg dose of extract shows hyper response in mice and fall of time of mice from rotating rod is an increase. As an indications of extract dose not showed Skeletal Muscle Relaxant Activity at higher dose. Conclusion: Methanolic Extract of Nerium Oleander posse’s significant muscle relaxant activity in comparing with control group. The muscle relaxant activity of extract may be due to its CNS-depressant activity due to cardenolides including a new cardenolides, neridginosides and three constitutents are nerizoside, neritaloside and odoroside –H.

Keywords: Nerium Oleander, Methanolic Extract, Rota Rod, Muscle Relaxant Activity, Mice etc.