Abstract

EXTRACTION, QUANTITATION & PURITY ESTIMATION OF GENOMIC DNA FROM AGERATUM CONYZOIDES L. LEAVES: EFFECT OF SEASON

Baibhav Karki, Ribek Thapa, Sudarshan Chettri, Tanaya Ghosh, Prasenjit Mitra, Prasanta Kumar Mitra*

Abstract

Isolation of pure DNA from the plant sources is important for genetic study leading to identification of the respective plant. In the present investigation attempts have been made for the first time to isolate pure DNA from Ageratum conyzoides L.(A. conyzoides L.) leaves. As season has effect on synthesis of chemicals in the plant parts, attempts have also been made to study the seasonal effect on concentration of pure DNA in A. conyzoides L. leaves. During autumn, winter, summer and rainy seasons leaves of A. conyzoides L. were collected, identified by expertsand the leaves were processed for extraction, quantitation & purity estimation of genomic DNA by the conventional methods. Results showed that pure DNA in high amount was isolated from A. conyzoides L. leaves during rainy season (June – August). It is concluded that A. conyzoides L. leaves of rainy season may be used to get high amount of pure DNA.

Keywords: Ageratum conyzoides L., DNA extraction, Purity estimation, Effect of season.