Abstract

EFFECT OF RESVERATROL ON LIVER DIFFERENTIAL HEPATOCYTES AND KIDNEY HISTOPATHOLOGY ON LEADINDUCED TOXICITY IN WISTAR RATS

Salisu Muhammad Highab*, Rabiu Abdussalam Magaji, Musa Aliyu, Nuhu Muhammad Danjuma and Bala Yauri Muhammad

Abstract

The aim of this experiment was to investigate the effect of resveratrol on liver differential hepatocytes and kidney histopathology in leadinduced toxicity in wistar rats. The study employed wistar rats (150 - 200 g) which were administered carboxymethylcellulose 10 g/l (control), lead acetate solution (120 mg/kg), lead acetate solution (120 mg/kg) and succimer (10 mg/kg BW); lead acetate solution (120 mg/kg) and resveratrol (200 mg/kg); lead acetate solution (120 mg/kg) and resveratrol (400 mg/kg); and resveratrol alone (400 mg/kg) then administered lead acetate solution (120 mg/kg) daily for 2 weeks and considered as prophylactic group. All treatments were through the oral routes by gavage. The organ samples were examined for histopathology. In the histopathology, the toxic effect of lead recorded in liver differential hepatocytes and kidney in the positive control group were significant (p < 0.05) when compared to resveratrol-treated groups. The toxic effects caused by lead acetate were reduced to minimal level in resveratrol-treated groups. Resveratrol (200 mg/kg) treated rat’s liver showed a marked improvement in the pattern of glycogen storage in hepatocytes. Resveratrol (400 mg/kg) treated rats’ liver showed a marked improvement in the pattern of glycogen storage in hepatocytes more than as seen in resveratrol (200 mg/kg). Resveratrol pretreated rats’ liver showed a marked improvement in the pattern of glycogen storage in hepatocytes more than as seen in resveratrol (400 mg/kg) treated rats. Rats treated with resveratrol (200 mg/kg) showed improved renal cortex histology, thus, no significant histopathology seen. Rats treated with resveratrol (400 mg/kg) showed well preserved renal cortex histology. Thus, no significant histopathology was seen. Rats pretreated with resveratrol (400 mg/kg) showed the same findings as that seen in resveratrol treated rats. In conclusion, resveratrol improved the adverse effects induced by the lead acetate in the liver differential hepatocytes and kidney tissues of the wistar rats.

Keywords: Resveratrol, Wistar Rats, Liver Differential Hepatocytes, Kidney, Lead Toxicity.