Abstract

IN VITRO ANALYSIS OF ANTIFUNGAL ACTIVITY OF ALOE VERA EXTRACT AND ALOIN ON CANDIDA ALBICANS

Shobhna Tiwari* and Dr. M. K. Gupta

Abstract

The aim of the study was to analyse, antifungal activity of Aloe vera extract and aloin on candida albicans. 4 different solvents were used for the extraction of Aloe vera which were extracted in petroleum ether, chloroform, ethyl acetate and 70% methanolic extract. Antifungal activity was tested by well diffusion assay. To determine minimum inhibitory concentration(MIC), agar dilution and broth dilution methods were performed. Each extract was taken in 4 varying concentrations (100mg/ml, 150mg/ml, 200mg/ml and 250mg/ml) to analyse the MIC by agar dilution method, which were further tested by broth dilution method. Among extracts, 70% methanolic extract shows maximum inhibitory action and extracts were compared with aloin which has shown more potent antifungal activity than all the extracts, it is active ingredient of Aloe vera. To compare the results fluconazole was taken as standard.

Keywords: Aloe vera, aloin, MIC, agar dilution, tube dilution.