Abstract

SCREENING OF THE ANTI-INFLAMMATORY ACTIVITY OF SIMAROUBA GLAUCA EXTRACT AGAINST FORMALDEHYDE INDUCED INFLAMMATION IN RATS

Nawres Taha Abdullah*, Nader Hamoud Khair and Raju Koneri

Abstract

Objective: The aim of the current study was to evaluate in-vivo Anti- Inflammatory activity of Simarouba glauca against formaldehyde induced inflammation in Wistar albino Rats. Methods: The methanolic exctract of Simarouba glauca was observed in formaldehyde (acute)- induced hind paw oedema test in rats. The Simarouba glauca were dried under shade and then powdered, and extracted with 90% methanol by reflux. Preliminary phytochemical studies and acute toxicity studies were also carried out. Inflammation was induced in the left hind paw of Wistar rats by injecting 0.1 ml of 4% formaldehyde solution below the plantar aponeurosis of the hind paw of the rats in the last day of the experiment. Group 1 served as normal control while group 2 was considered as disease control. Group 3 was standard receiving Diclofenac 10 mg/kg and Groups 4 and 5 disease animals were treated with Simarouba glauca (250 mg/kg and 500 mg/kg respectively). The parameters included in this study was to observe the inflammation of the paw edema of rats. Results: The present study demonstrated that Simarouba glauca extract has an anti-inflammatory action against the acute phase of inflammation using the Plethysmometer. Interpretation and conclusion: Significant Antiinflammatory activity of Simarouba glauca observed in the present investigation could be the result of reducing the inflammation. Methanolic extract of Simarouba glauca also showed improvisation in reducing the inflammation and may have protective effect in Antiinflammatory related complications.

Keywords: Simarouba glauca, Anti-inflammation, Formaldehyde, Plethysmometer.