Abstract

IN VITRO FREE RADICAL SCAVENGING ASSAY (DPPH (2, 2- DIPHENYL 1-2 PICRYLHYDRAZYL METHOD) OF SIDDHA HIV HERBAL FORMULATION DEVA CHOORNAM

Thangadurai K.*, Rengasundari R., Vinayak S., Gayatri R., Suresh K. and Banumathi V.

Abstract

Background: Siddha Herbal powder formulations like Choornams, are now well accepted as a supportive therapy for most of the life style diseases and other prevalent ailments in our society owing to its considerable antioxidant property for quenching harmful free radicals formed inside the body. Deva Choornam, a poly herbal formulation has been successfully implemented for improving the Quality of life in Immuno compromised conditions especially AIDS. Objectives: The study aims to analyze the free radical scavenging activity of Deva choornam (DC) against DPPH (2,2-diphenyl 1-2 picrylhydrazyl) Free radical. Methods: The Choornam were prepared as with reference to Siddha texts. Reaction mixture containing 1 ml of 0.3 m M DPPH methanol solution was added to 2.5 ml of sample solution of Deva choornam and standard solution of Ascorbic acid of different concentrations and allowed to react at room temperature. Absorbance in the presence of test sample DC at different concentration of (10 μg, 20 μg, 40 μg, 60 μg, 80 μg and 100μg/ml) was noted after 15 min incubation period at 370C. Absorbance was read out at 517 nm using double-beam U.V Spectrophotometer by using methanol as blank. Results: The effective concentration of test sample DC required to scavenge DPPH radical by 50% (IC50 value) was obtained as 73.24 ± 18.47 in compare with Ascorbic acid (13.88 ± 0.93). Conclusion: Deva choornam exhibited moderate Anti oxidant property as compared with the standard and thereby validating a supportive role in its successive clinical applications in HIV for improving the quality of life.

Keywords: Siddha medicine, HIV, Deva Choornam, DPPH free Radical scavenging Activity, Anti Oxidant.