Abstract

STUDY OF BIO-LARVICIDAL ACTIVITY ON AEDES AEGYPTI THROUGH BIOASSAY BY CRUDE EXTRACT OF DIFFERENT PARTS OF CARICA PAPAYA PLANT ALONG WITH AN IN-SILICO APPROACH FOR TOXICITY AND MUTAGENICITY OF ESTABLISHED PHYTOCHEMICALS BY USING QSAR MODELLING

Partha Talukdar*, Nandini Ghosh and Sumitra Verma

Abstract

Carica papaya Linn is a common tree, and containing phytocompounds for medicinal and bio-larvicidal properties. The objective of the present study was to detect percentage mortality of larvae of Aedes aegypti by aqueous extract of different parts such as leaf, seed, unriped fruit, latex and flower of C. papaya and in silico predictive study for toxicity and mutagenicity of established phytochemicals of C. papaya through QSAR modelling. Bioassay experiment was done with different parts of aqueous extract with following dilutions viz. 20%, 40%, 60%, 80% and 100% respectively and % mortality was observed in A. aegypti for 24h and 48h durations. Next, QSAR modelling was done to detect toxicity in D. magna, P. promelas, rat oral and mutagenicity for established phytochemicals and the confirmation of phytochemicals was done by TLC and NMR. The present study revealed that phytochemicals of C. papaya leaf, seed and unriped fruit extract have highest ability to destroy mosquito larvae of A. aegypti compared to latex and flower extract. Also, QSAR modelling revealed phytocompounds such as Benzylisothiocyanate, Quercetin, Hentriacontane, Carpaine, and Linalool showed higher toxicity in D. magna while in P. promelas highest toxicity found in this manner as Linalool >Benzylisothiocyanate> Carpaine>Quercetin>Hentriacontane. It was also observed that two phytocompounds such as Benzylisothiocyanate and Quercetin were mutagenic positive others found negative. Quercetin was confirmed both TLC and NMR study. In conclusion, studied phytochemicals from crude extracts can be isolated and prepared bio-larvicide from each phytocompound prior to functional assay.

Keywords: Carica papaya; Phytochemicals; Bioassay of extracts; Predictive toxicity and mutagenicity; QSAR modelling; In silico study.