Abstract

THE EVALUATION OF CARDIOPROTECTIVE ACTIVITY OF ECKLONIA CAVA EXTRACT IN RATS

Nader Hamoud Khair*, Kusu Susan Cyriac, Raju Koneri and Nourhan Abubakr Elsayed Barakat

Abstract

Acute myocardial infarction is an important ischemic heart disease and a leading cause of morbidity and mortality worldwide. Isoproterenol (ISO) is a synthetic β-adernoceptor agonist that its injection induces myocardial infarction in rats. The goal of this study was to evaluate the cardioprotective effects methanolic extract from a brown algae Ecklonia cava (EC) against ISO-induced cardiotoxicity in a rat model. A total of 24 rats were divided into four groups: normal, control, lowdose EC, high-dose EC. Biochemical parameters were measured. The blood was subjected to centrifugation to isolate serum out of it. The serum was further used for estimation of CK-MB, Lactate Dehydrogenase (LDH), Alanine transaminase (SGPT), Aspartate transaminase (SGOT), Total Cholesterol (TC), Triglycerides (TG), LDL, and HDL for Cardioprotective activity. And Antioxidant Enzymes Superoxide dismutase SOD and Choline acetyltransferase CAT of heart tissue were measured to determine the antioxidant activity. Hearts were isolated were observed under light microscope for histopathological study. Methanolic extract of Ecklonia cava treated group showed significant reduction in CK-MB, LDH, SGOT, SGPT, TG, TC, LDL and increase in HDL levels when compared to toxic control group, as well as increase in the activities of antiperoxidative enzymes SOD, CAT in the heart tissue and was further confirmed by histopathology study.

Keywords: Ecklonia cava, Cardioprotective, Isoproterenol.