Abstract

OXIDATIVE SPECTROPHOTOMETRIC DETERMINATION OF COMMERCIAL DRUGS USING POTASSIUM PERMANGANATE AND AMARANTH DYE AS A COUPLE IN ACIDIC MEDIUM

Mamidi Gopi and G. Venkateshwarlu*

Abstract

Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Cefdinir (CEF), Ceftriaxone (CET), Doxycycline (DOX), Losartan (LOT) and Ranitidine HCl (RAN), based on their reactivity towards Acidic Potassium Permanganate (KMnO4). Beers law is obeyed in the concentration of 3-27 μg mL-1, 4.5-31.5 μg mL-1, and 6.5-45.5μg mL-1 and 10-70 μg mL-1., 8-56 μg mL-1 for CEF, CET, DOX, LOT and RAN respectively. The methods involve the addition of excess KMnO4 of known concentration in the presence of 2N Sulphuric Acid (H2SO4), Reactants are allowed to react and the unreacted KMnO4 is estimated by the measurement, linear relationship is observed between absorbance of Amaranth dye (λmax =519nm) and Concentration of drug and formed a basis for quantification. These methods have been applied for the determination of drugs in their pure form as well as in tablet formulations. These methods have been validated in terms of guidelines of ICH.

Keywords: Spectrophotometry, Drugs, KMnO4, Amaranth dye, Determination, Validation.