Abstract

DEVELOPMENT OF RAPD POLYMORPHIC DNA BASED SCAR MARKER FOR IDENTIFICATION OF THESPESIA POPULNEA – A BARK DRUG USED IN AYURVEDA

*Mallya Suma V.

Abstract

Introduction: Thespecia populnea (L.)Sol.ex Corr, one among Panchavalkala, the bark of which is used medicinally in various diseases. Because of similar appearance of bark drugs, quality standard parameters are essential requisite. Molecular markers are like DNA SCAR markers are most reliable methods of quality parameters. Hence an attempt made to generate DNA SCAR markers of bark drug T. populnea. Materials and Methods: Authenticated bark samples of T. poppulnea were collected, along with samples of Panchavalakala. DNA extraction carried out through CTAB method and purified. Templates of all five species of Panchavalkala were used for RAPD. Based on RAPD results, unique fragments were selected for SCAR marker development. Results: Unique band of ~500bp was observed for Thespesia populnea which was not seen in other species. This fragment was gel purified, amplified and used for cloning into T vector. Clones were confirmed by amplification using vector primers and was sequenced. Sequence obtained was analysed and primers were designed to give ~450bp product for this region and reconfirmed.

Keywords: Thespecia populnea (L.), Panchavalakala.