Abstract

ISOLATION, CHARACTERIZATION & BIOCHEMICAL ANALYSIS OF MULTIDRUG-RESISTANT BACTERIA FROM SOIL SAMPLES COLLECTED FROM HOSPITAL WASTE AND THEIR MOLECULAR CHARACTERIZATION USING 16S RDNA

Iqbal Hussain Mir*, Puja Baba, Pratibha Singh, Dr. Mukesh Sharma and Ajeet Singh

Abstract

The antibiotic sensitivity test was performed for the isolated cultures obtained from different hospital waste of Delhi & NCR. During the study, out of 25 cultures, only 5 samples showed a remarkable antibiotic resistance. The bacterial isolates were characterized biochemically with the help of Bergey’s manual. They included; Neisseria mucosa, Staphylococcus epidermidis and Morecoccus cerebrosus. The bacterial isolates were further confirmed by molecular characterization using 16s rDNA. The total of 11 antibiotics were used included; Tetracycline, Ampicillin, Sparfloxacin, Co-timoxazole, Gatifloxacin, Cefrizoxime, Amoxicillin, Ciprofloxacin, Nitrofurantoin, Ofloxacin and Streptomycin. All strains were found resistant to Tetracycline, Ampicillin Sparfloxacin, Co-timoxazole and Gatifloxacin. 8 antibiotics showed an intermediate resistance. All isolates were found sensitive to Nitrofurantoin, Ofloxacin and Streptomycin.

Keywords: MDRO’s, Hospital waste, Antibiotics, 16s rDNA.