Abstract

HISTOPATHOLOGICAL AND BIOCHEMICAL EFFECTS OF PARACETAMOL-INDUCED TOXICITY IN FRESHWATER FISH LABEO ROHITA

D. Siva Shanmuga Raja and U. Dhivya Bharathi*

Abstract

Recently, Chemical toxicity has become one of the major problems in aquaculture; many farms have been suffering from the “liver syndrome”, with symptoms of liver enlargement (up to two to threefold of the original size) and colour change. The causes of this disease are not clear; pathogenic bacteria or viruses have not been identified. Xenobiotic challenge due to drug abuse and environmental pollution may be one of the most important causes of the diseases. Liver lesions have been reported in various fish species exposed to different xenobiotics. Biological compounds with antioxidant properties contribute to the protection of cells and tissues against the deleterious effects of reactive oxygen species and other free radicals. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in the regeneration of hepatic cell. The present study aimed to evaluate the Histopathological and biochemical effects of paracetamol-induced toxicity in freshwater fish Labeo rohita. The following conclusion obtained after supplementation of paracetamol exposed fish. Increased the oxidative stress confirmed by the formation of MDA. Declined the antioxidant enzymes GSH, SOD, Catalase and GPx. Increased the liver markers ALT and AST. Decline the protein content in liver, gill and muscles. Histopathalogical observations confirmed its hepatotoxic activity. Over all, the protective effect of paracetamol-induced hepatotoxicity in fishes appears to be related to the increased of lipid peroxidation and decline of antioxidant enzyme levels in addition to free radical generation.

Keywords: Histopathology, Biochemical, Antioxidant, liver markers, Labeo rohita.