Abstract

DOSE-RESPONSIVE SYSTEMIC TOXICITY OF CYPERMETHRIN IN WISTAR RATS

Anurag Paramanik, Tuhina Das, Rini Ghosh, Ananya Pradhan, Subhabrata Das, Suman Mondal, Prasanta Maiti and Sujata Maiti Choudhury*

Abstract

Pesticides are used frequently and have various adverse effects on human health in different ways. Cypermethrin (CYP), a synthetic pyrethroid, is used extensively to control a wide variety of pests in agriculture, forestry, horticulture, and public health. This study designed to investigate the dose-dependent gonadal, immune and systemic toxicity of CYP in mature male and female Wistar rats. Rats were randomly divided into nine groups, different doses (1/11th, 1/10th, 1/9th, 1/7th, 1/6th, ¼.5th, 1/4th, 1/3rd incase of male rat; 1/11th, 1/10th, 1/9th, 1/7th, 1/6th, 1/5th, 1/4th incase of female rat) of CYP were administered for 14 consecutive days and different gonadal, immune and systemic parameters were assessed. Sperm viability, testicular acid phosphatase, WBC count, lymphocyte count, cerebellar and cerebellum reduced glutathione (GSH) content were significantly diminished. Serum urea, creatinine, cerebellar and cerebellum malondialdehyde (MDA) content were increased following CYP treatment in male rats at 40 and 80 mg/kg body weight (1/9th and 1/4.5th LD50). Decreased SOD and GST levels were also observed in CYP-exposed female rats at a dose level of 34.33 and 51.5 mg/kg body wt. (1/9th and 1/6th LD50). In conclusion, cypermethrin induced gonadal, immune and systemic toxicity in mature male rats with a body weight of 40 mg/kg (1/9thLD50) and gonadal toxicity in mature female rats with a body weight of 34.33 mg/kg (1/9thLD50) and above.

Keywords: Cypermethrin; Gonadal; Immune; systemic; Testicular ACP; Cerebellar MDA.