Abstract

BIOPRODUCTION OF INDOLE 3-ACETIC ACID BY TRICHODERMA STRAINS ISOLATED FROM AGRICULTURE FIELD SOILS IN SENEGAL

Ndiogou Gueye, Kranthi Kumar G., Adiouma Dangue, Mame Arama Fall Ndiaye, Tahir A. Diop and M. Raghu Ram*

Abstract

A total of twenty Trichoderma strains were isolated from rhizospheric soils of tomato fields from different areas of Niaye zone, main area of horticulture production in Senegal. Identification of these Trichoderma isolates was based on morphological and cultural characters on potato dextrose agar medium. Of the 20 strains, only 8 strains showed the maximum indole acetic acid production. Among the 8 strains TG 4 showed maximum (90 μg/ml) indole acetic acid production was selected for optimization studies. Optimum conditions for maximum IAA were 8 days of incubation and pH 7.0 at 37⁰C temperature. Among the carbon and nitrogen sources tested, Mannitol and yeast extract induced the maximum IAA production of 105μg/ml and 95μg/ml respectively. Possession of plant growth promoting traits like Trichoderma TG 4 strain a promising strain to be developed as multifunctional biofertilizer.

Keywords: Trichoderma, Indole acetic acid (IAA), Potato dextrose agar (PDA).