Abstract

ACUTE ORAL TOXICITY AND HISTOPATHOLOGICAL EVALUATION OF SIDDHA DRUG MAHA ANALURUVA CHOORANAM (MAC) IN WISTAR RATS – A STUDY

A. Janakiram*, J. Prabhavathi, Prof. Dr. S. Kaniraja, Dr. N. J. Muthukumar and Prof. Dr. V. Banumathi

Abstract

In Siddha system of traditional medicine, Maha analuruva Chooranam (MAC) is an Herbal formulation. In the reference text of “Agasthiyar vaithiya vallathi 600”, it has been used for the treatment of Kumbavatham (periarthritis of shoulder or Adhesive capsulitis or frozen shoulder). Kumbavatham is a condition which deals with the involvement of painful and restricted movements of the shoulder joint, a kind of arthritis attended with boring pain in the shoulders and arms and restricted movements of the upper limb. Siddha medicine MAC is a good remedy for the periarthritis of shoulder. In this study the author has aimed to evaluate safety and efficacy of the trail drug MAC in female Wistar rats (Nulliparous, Non-Pregnant). Acute oral toxicity was performed as per organization for economic co-operation for the development (OECD) guideline 423 methods. Acute toxicity studies were carried out in 5 groups of 15 female Wistar rats, a dose of 100mg, 250mg, 500mg, 1000mg; 2000mg of MAC was administered and monitored for any toxicity effects. After 14 days the animals were sacrificed and the histopathological analysis of the liver, lung, kidney, spleen, of treated groups did not show any sign of toxicity. Maha analuruva chooranam was Non-toxic in acute toxicity study.

Keywords: Siddha System, Maha analuruva chooranam, Acute Oral Toxicity, Histopathology, Wistar rats.