Abstract

ANALYTICAL STANDARDIZATION OF GANDHAKAKALPA

V. Shyamaladevi*, Rameshbabu G., Venkatasubbaiah K. and Ch. Sridurga

Abstract

Rasa shastra is a branch of medicine which deals with preparation of drugs of metals and minerals having wide range of therapeutic efficacy and possessing innate qualities like quick action, less dose, tastelessness, prolonged shelf life and better palatability. Gandhakakalpa is one of the unique formulations in which Shuddha Gandhaka and Madhuka taila are the main ingredients. Shodhana, Murchchana and Mardana are the main pharmaceutical procedures involved in the preparation of Gandhakakalpa. To assure the safety and to understand about the identity, form, particle size and surface morphology of the above formulation, it was subjected to analysis through various analytical parameters like X-ray diffraction (XRD), Scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDS), Particle size analysis (PSA), Zeta Potential (ZP), UV-Spectroscopy, Fourier transform InfraRed spectroscopy (FTIR) and Inductively Coupled Plasma – Optical Emission Spectrometry (ICP-OES). XRD of Gandhakakalpa shows major peaks of S8. SEM micrographs showed spherical and glomerulated particles; particle size was found to be 14.7nm and its Zeta potential is -32.7 mV, UV spectrum of Gandhakakalpa showed maximum absorption at 261nm and 268nm, FT-IR analysis showed 11 peaks between the wavelengths 3006.72 – 407.54 cm-1 and ICP – OES analysis revealed Sulphur as main constituent in 96164 ppm.

Keywords: Gandhakakalpa, Modern Analytical parameters, Safety, Toxicity.