Abstract

ANTIUROLITHIATIC ACTIVITY OF VITIS VINIFERA ON ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATS

Chandana Edla* and Dr. Pradeep Kumar Challa

Abstract

Introduction: Urolithiasis is a common problem afflicted for centuries with high recurrence and is predominant in males with no satisfactory method available for complete cure. Vitis vinifera is a plant commonly used as a traditional herbal medicine and posses the wide range of pharmacological applications. Objective: The present study is to investigate the anti-urolithiatic activity of Vitis vinifera against ethylene-glycol induced urolithiasis in rats. Methods: Urolithiasis was induced by oral administration of 0.75%v/v ethylene glycol and 2%w/v ammonium chloride in drinking water for 21days. 20 Male Albino rats were randomly divided into 5 groups. Group-1 received normal drinking water. Group:2-5 received ethylene glycol and ammonium chloride for 21days. Group 3&4 received leaf extract of Vitis vinifera at oral dose of 200mg/kg & 400mg/kg respectively from 1st day to 21st day. Group 5 received standard drug cystone(750mg/kg p.o). After completion of 21days respective treatments, the levels of various urolithiatic parameters in the urine, serum and kidney homogenate were used as criteria for assessing antiurolithiatic effect of Vitis vinifera. Results: The urolithiatic control group showed significant decrease in urine volume. The uric acid, calcium and creatinine levels were increased. Rats treated with vitis vinifera leaf extract significantly decreased the urinary excretion of calcium, serum creatinine and uric acid levels. Histopathological studies confirmed the crystal deposition in sections of kidney from animals treated with ethylene glycol and this was reduced in treatment with leaf extract. Conclusion: These results indicate that Vitis vinifera leaves are effective against ethylene glycol induced urolithiasis.

Keywords: Urolithiasis; ethylene glycol; ammonium chloride; Vitis vinifera; cystone; calcium oxalate.