Abstract

EFFECT OF KINETIN CONCENTRATION ON AXILLARY SHOOT BUD PROLIFERATION OF BAMBUSA VULGARIS

Charu Rajpal*, Akansha Kanaujia and Pushpa C. Tomar

Abstract

Bambusa vulgaris is a widely cultivated and fastest growing woody species of Poaceae family that plays an important role in day to-day life of million people. As this species does not produce seeds after sparse flowering so vegetative propagation is an exclusive alternative that makes progeny available, so a few low cost propagation trials were conducted for the clonal propagation of these species by inter-nodal branch cuttings. These cuttings were then treated with different concentration of Kinetin (2 mg/l, 3 mg/l, 4 mg/l, 5 mg/l, 6 mg/l) and kept in plant growth chamber to assess shoot bud proliferation. These explants were kept under observation for 4 weeks and then subcultured with MS-Media for shoot proliferation. Then these subcultured plantlets were acclimatized and regularly cultured with Hoagland Media at specific time intervals. However, no growth was observed when explants were treated with antibacterial (Streptomycin sulphate, 0.0015 gms/l) and antifungal (Bavistin, 0.2%) treatment. The length of the longest shoot varied dependent of branch cutting types and concentrations of Kinetin solutions. This study concluded that inter nodal branch cuttings are able to develop shoots under greenhouse conditions. Cost effective and high quality plantlets were obtained in minimum time through this process of micro propagation.

Keywords: Bambusa vulgaris, Micro Propagation, Explants, MS-Media, Plant Growth Regulators.