Abstract

COMPARATIVE STUDY ON THE EFFECT OF NANO-CURCUMIN COMPLEXES AS ANTITUMOR IN MICE

Radwa Wahid Mohamed, Tahani Elsayed Mohamed, Gehan Salah Eldin Moram*,
Abdelfattah Mohsen Badawy and Mohamad Fahmy Zaky

Abstract

This study was undertaken to compare the antitumor activity of copperoxide nanoparticles (CuO-NPs), hydrazine sulphate-coppernanoparticle (HS-Cu-NPs), nano-curcumin (Nano-Cur), curcumincapped copper nanoparticles (Cur-Cu-NPs) and native curcumin(Native-Cur) with ascorbate in vivo and in vitro using breast carcinomacell line (MCF-7). Methods: CuO-NPs and HS-Cu-NPs were injectedintratumorally at dose 10 mg/Kg b.wt., Cur-Cu-NPs was injectedintratumorally at dose 50 mg/Kg b.wt., Nano-Cur and Native-Cur wereinjected intratumorally (G6, G7) or administered orally (G8, G9) atdose 50 mg/Kg b.wt. In vivo, Female mice were divided into 9 groups:G1: Healthy control. G2: tumor bearing mice (TBM). G3:TBM+CuO-NPs + ascorbate. G4: TBM+ HS-Cu-NPs + ascorbate. G5: TBM+Cur-Cu-NPs +ascorbate. G6, G8: TBM+Nano-Cur + ascorbate G7, G9: TBM+Native-Cur +ascorbate. Invitro, Cell lines were treated with the different nano complexes for 48h. Results: In vivo,Different nano-complexes significantly reduced tumor markers and inflammationimmunologic markers and significantly elevated the caspase-3 activity and P53 geneexpression. These results were confirmed by histopathological examination andimmunohistochemistry. In vitro, all nano-complexes showed a clear cytotoxic effect on(MCF-7). In conclusion: All nano-complexes administration significantly inhibited tumorgrowth and reduced tumor markers but Cur-Cu nano-complex was the most effectivetreatment.

Keywords: Curcumin, Nanocurcumin, Curcumin capped copper nanoparticles, EAC, tumor bearing mice.