Abstract

IN-VITRO ASSESSMENT OF ANTIOXIDATIVE POTENTIALS OF ETHANOL SEED EXTRACT OF COLA LEPIDOTA K. SCHUM

Nwankpa P.*, Chukwuemeka O. G., Ekweogu C. N., Ugwuezumba P. C, Etteh C. C., Ngwu E. E., Emengaha F. C. and Egwurugwu J. N.

Abstract

The aim of this study was to carry out an in-vitro assessment ofantioxidative potential of ethanol seed extract of Cola lepidota K.Schum. In-vitro determination of antioxidant property of seed extractof Cola lepidota was done using ferric reducing antioxidant power(FRAP), 2,2-dyhenyl-l-picrylhydrazyl (DPPH) assay and nitric oxideradical (NO) scavenging activity. These assessments were carried outusing standard antioxidant assays. Results obtained from theantioxidant assay show that the extract of Cola lepidota seed has aninsignificant ferric reducing power (p≥0.05) (0.01, 0.05, 0.10, 0.20,0.50μM) when compared to the ascorbic acid control (0.1, 0.25, 0.75,1.50, 1.75μM). However, DPPH and NO radical scavenging ability of the seed extract (20.50,36.20, 60.04, 92.20, 92.30 and 36.42, 58.00, 60.24, 62.10, 66.40 respectively) wasstatistically significant p≤ 0.05 when compared with the standard drug ascorbic acid 92.10,94.00, 94.06, 94.05, 96.06 and 66.21, 71.06, 90.22, 94.88, 97.21 respectively, at25,50,100,200 and 400 mg/ml. In conclusion, the result of this study lends credence to aprobable role of the seeds of Cola lepidota as a potent antioxidant.

Keywords: Cola lepidota seed, DPPH, NO., FRAP, antioxidant potential.