Abstract

HPLC ANALYSIS OF WITHANOLIDES IN HEAVY METAL TREATED PLANT DRUG PARTS (LEAVES AND ROOTS) OF WITHANIA SOMNIFERA (L) DUNAL.

Ch. Saidulu*, C. Venkateshwar, S. Gangadhar Rao

Abstract

Plants were grown in pot culture experiments with three treatments in black soil, Treatment No I. a control without any addition to the soil, Treatment No II Cadmium 10ppm, Chromium 20ppm, Nickel 16ppm were introduced into the soil, Treatment No III one % of Calcium hydroxide was also added along with heavy metals to soil. Then plants were grown up to the productivity levels. Withania somnifera roots, leaves and preparations of the plant are traditionally used as tonic, sedative and diuretic. It is also having a high medicinal value and possesses potent anti-tumor and antioxidant properties. Withania somnifera which mainly contains Withanolides are which biologically active secondary metabolites present in roots and leaves. In the present study the maximum concentration of Withanolide A was observed in the roots and in the leaves of in the control plants (Treatment I) when compared to the other two treatments. Withanolide A concentration is almost same in heavy metal treated plants (Treatment II) and heavy metal +1% Ca (OH)2 treated (Treatment III) plants. The roots showed Withanolides A more concentration when compared with leaves in three treatments. The concentration of Withanolies A of roots and leaves in three treatments there was no significant differences. So leaves can also be used as the raw material for the extraction of Withanolide A from Withania somnifera.

Keywords: Withania somnifera, Roots and Leaves, Withanolide A, Heavy metal treated plants, High Performance Liquid Chromatography, Crud drugs.