Abstract

IN-VITRO EVALUATION OF ANTI-MICROBIAL POTENTIAL OF TRADITIONAL SIDDHA FORMULATION SEENTHIL CHOORANAM AGAINST UROPATHOGEN BY DISC-DIFFUSION TECHNIQUE

J. Nisha*, N. J. Muthu Kumar, S. Visweswaran, N. Anbu and V. Banumathi

Abstract

Urinary tract infections (UTIs) are one of the most frequent clinical bacterial infections in women, accounting for nearly 25% of all infections. Around 50–60% of women will experience a UTI in their lifetime. Increasing antimicrobial resistance has stimulated interest in non-antibiotic prophylaxis of recurrent UTIs. Well-known steps in the pathogenesis of UTIs are urogenital colonization and adherence of uropathogens to uroepithelial cell receptors. Escherichia coli (E. coli) is an opportunistic pathogens that cause severe and life-threatening infections in immunocompromised patients. In women with recurrent UTIs an increased adherence of E. coli to urogenital epithelial cells was seen compared to healthy controls. Antibiotics are essential for the control and treatment of E. coli infections in humans and animals. However, it is generally accepted that antimicrobial resistance is associated with the quantity of antibiotic consumption. The worldwide emergence of multidrug-resistant bacteria has dramatically limited the number of antibiotics that retain activity against these pathogens. This problem has been further amplified by the dearth of novel classes of antibiotics. Therefore, development of novel therapeutic strategies for infectious diseases is high demand. Siddha system of medicine has excellent antimicrobial drugs with combination of bioactive components that’s acts synergistically in multiple way thereby chance of resistance is greatly reduced and also several siddha traditional drugs are been prescribed for treating UTI infection one such drug is Seenthil chooranam (SC). The main aim of the present investigation is to evaluate the anti-microbial potential of the SC against E-coli a known uropathogen by disc diffusion method. Results of the study has revealed that the drug SC at the concentration of 4000 μg exhibited the maximum zone of inhibition of 10mm when compare to that of the standard with the maximum zone of 12 mm. It was concluded from the data’s obtained from the present investigation that the therapeutic value of siddha formulation relies in the various chemical constituent's presents in it. The bioactivity of formulation SC may be attributed to phytochemical a constituent which needs to be studies in details in near future.

Keywords: Urinary tract infections, Escherichia coli, Antibiotic, Resistance, Antimicrobial, Siddha traditional drugs, Seenthil chooranam.