Abstract

METHOD DEVELOPMENT AND VALIDATION OF RESIDUES OF PESTICIDES IN RICE BY USING LC – MS/MS

SD. Rihana*, G. Sandhya Rani, G. Sumanjali, G. Jyothi and G. Chaitanya

Abstract

A rapid and sensitive method was developed and validated for the determination of around 100 pesticides in rice samples by using HPLC coupled to a triple quadruple detector mass analyser (LC-MS/MS) MS - (Agilent 6400) and LC -(Agilent 1290 infinity 2). The sample preparation step was optimized. Pesticides were extracted from rice samples by using ammonium formation, methanol, formicacid, water. It is a quick easy, cheap, effective, rugged and safe method was used for rice samples. The analytical method was optimized with a total run time of 10minutes with a flow rate of 0.800ml/min. By using Inertsil Column ODS-3V, 150×4.6mm, 5microns. The performance of the method was also evaluated in terms of linearity precision and accuracy and then it was applied to eight commercial rice samples from different suppliers. The results demonstrated the ability of the method to detect all the 20 pesticides with linearity, precision, and accuracy according to the protocols established by the most important organizations and validation guidelines.

Keywords: Tuning, LC-MS, Pesticides, Hexacanazole, Carbendazim, Clothiandin.