Abstract

ISOLATION AND CHARACTERISATION OFANTIMICROBIAL COMPOUNDS FROM LEAF EXTRACT OF ABUTILON INDICUM

Anitha Mary A.*, Santhi V. and P. Rani

Abstract

Medicinal plants are gifts of nature to cure limitless number of diseases among human beings. The production of medicines and the pharmacological treant of diseases began with the use of herbs. The leaves extracts Abutilon indicum and Morinda citrifolia were were taken the antimicrobial activity from the extracts of the plants Abutiln indicum was carried out in crude (ethyl acetate, methanol, chloroform) and column fractionated extracts (ethyl acetate (F1), ethyl acetate: chloroform (F2), chloroform (F3), methanol: chloroform(F4) & methanol(F5) against six bacterial and two human fungal pathogens Candida albicans and Aspergillus flaves using disc diffusion technique. Crude ethyl acetate, chloroform extract of A. indicum showed highest activity against S. flexneri (6mm) and crude methanol extract showed 6mm against V. cholerae. Among the tested pathogens F1 fraction & F5 fractions were well in inhibiting most of the tested pathogens. Among the crude ethyl acetate, methanol and chloroform extracts, methanol extract of A. indicum and chloroform extract of exhibited maximum inhibition zone against Candida albicans (22 mm) and column fractionated extracts F1 (ethylacetate) and F5 (methanol) fractions exhibited maximum inhibitory zone against C.albicans (11 mm). This study validated the use of this plant in folkloric medicines and a potential source of new classes of antibiotics that could be useful on control of human infectious diseases.

Keywords: Antifungal activity, inhibitory zone, GC-MS analysis, analgesic activity, anti-inflammatory activity.