Abstract

ISOLATION AND PURIFICATION OF LECTIN FROM PISUM SATIVUM

Prangya Paramita Acharya, Ajay Kumar Sahu* and Dr. Ditikrishna Sahu

Abstract

Lectins are glycoprotein or carbohydrate binding proteins having agglutinating property.Lectins were first found in plants and were later isolated from animals as well .interaction of lectines with the carbohydrates is very specific. Their specificity to particular sugar is defined by the mono or oligosaccharide that inhibits agglutination plant. Lectins have great potential as tools in identification, purification and stimulation of specific glycoconjugates. Leguminosae or fabaceae is the best characterized family of plant lectin. Other family of plant lectins includes Gramineae, solanaceae, euprobiaceae etc. Their function in plants include enzymatic activity and storage of protein defense mechanism, cell wall extension mutagenic stimulation, transport of carbohydrate and packaging and mobilization of storage materials .Pisum sativum lectin is a leguminous or fabaceae plant. Pea lectin shows specificity towards mannose or glucose. In the following report, it was the aim to isolate and purified the Pisum sativum lectin and its characterization by heamoagglutation assay.

Keywords: Isolation, purification, lectin, Pisum Sativum, SDS- PAGE, Lowry method.