Abstract

IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF CANTHIUM CORAMANDELIUM EXTRACT

*Dr.V.P.Karthik, Dr.Srinivasan, Dr.Gowri, Dr.Punnagai

Abstract

Introduction: CanthiumCoramandelium is a shrub widely indicated for dysentery. An antioxidant is molecule that inhibits the oxidation of other molecules. Antioxidants are widely used in dietary supplements and have been investigated for the prevention of diseases such as cancer, coronary heart disease and altitude sickness. In this study we have attempted to evaluate the free radical scavenging activity of CathiumCoramandelium using in-vitro 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay. Objective:The objective of the present study was to evaluate the free radical scavenging activity and antioxidant potential of Canthium Coramandelium. Material & methods: DPPH radical scavenging activity was done using the method of Modified method of Bios (1985). The reaction mixture containing 1ml of DPPH solution (1mM in ethanol) with different concentrations of the substance (20, 40, 60, 80, μg/1ml) was shaken and incubated in dark for 20 min at room temperature. The resultant absorbance was recorded at 517nm using spectrophotometer. The percentage inhibition was calculated using the formula Percentage inhibition = Abs control- Abs sample * 100. Abs control Results:At concentrations of 20, 40, 60, 80 μg/ml percentage of inhibition observed was 10.36±0.18, 31.98±0.54, 68.07±0.19 and 91.02±0.04 respectively. Discussion & conclusion: Thus this invitro study suggest that CanthiumCoramandelium possesses antioxidant properties. Prior phytochemical analysis CanthiumCoramandelium shows the presence of flavonoids, tannins and glycosides. The antioxidant property of CanthiumCoramandelium may be attributed to the presence of flavonoids and tannins. However pharmacokinetic and safety profile of Canthium Coramandelium requires pre-clinical testing prior to its clinical application in human beings.

Keywords: Canthium Coramandelium, Antioxidant, DPPH assay.