Abstract

IN SILICO DOCKING STUDIES ON SGLT 2, FOXO1, GLYCOGEN SYNTHASE ACTIVITY BY ISOLATED ACTIVE PRINCIPLES OF STEREOSPERMUM TETRAGONUM DC

Bino Kingsley*, Anjali, Apian Subramoniam and Brindha Pemiah

Abstract

Background: Stereospermum tetragonum is a potential source of metabolites such as coumarines, glycosides, tannins, terpenoids and traditionally used in the treatment of Diabetes mellitus. The present work attempts to evaluate the interaction of active principles evaluated from S. tetragonum with SGLT 2, FOXO1, Glycogen Synthase by insilico docking studies. Methods: Compounds isolated from active fraction of water extract of S. tetragonum which are analyzed by spectral studies and identified as1,4a,5,7a-tetrahydro-5-hydroxy-7- (hydroxymethyl)-1-(tetrahydro-6-(hydroxymethyl)-3,4,5-trimethoxy- 2H-pyran-2-yloxy)cyclopenta[c]pyran-4-carboxylicacid and 5,8- dihydro-7-isopentyl-2,3,5,8-tetramethoxynaphthalene-1,4,6-triol. The two bioactive molecules were active in lowering the blood glucose level. These bioactive molecules is activated with SGLT 2, FOXO1, Glycogen synthase by insilico docking studies. The two bio active molecules were active in lowering the blood glucose level at 2mg/kg dose. Results: The isolated compounds showed better interaction than standard metformin through an extensive insilico docking approach with SGLT 2, FOXO1, Glycogen synthase with the receptors. The glide score for SGLT 2 for compound 1 is -8.765 for compound 2 is -7.056 and for metformin -1.711. In FOXO1 the glide score for standard metformin -0.381 and for compound 1 is -4.377 and compound 2 is -2.715. In glycogen synthase the glide score of compound 1 is -7.558, compound 2 is -5.454 and standard metformin is -3.39 with the receptors. Conclusion: The work establishes the isolated compounds from the fraction of S. tetragonum as a potential source for diabetes mellitus thus enabling a possibility of this plant as new alternative to existing diabetic approaches.

Keywords: SGLT 2, FOXO1, Glycogen Synthase Stereospermum tetragonum.