Abstract

STUDY ON ANTIOXIDANT CAPACITY AND ANTICANCER ACTIVITY OF BISMILLAH LEAF (VERNONIAAMYGDALINA).

Mohd Adzim Khalili Rohin*, NorhayatiAbd Hadi, Rochman Naim, Atif Amin Baig and Khairil Mahmud

Abstract

Vernoniaamygdalina is commonly called bitter leaf because of its bitter taste. The leaves may be consumed either as a vegetable (macerated leaves in soups) or aqueous extracts as tonics for the treatment of various illnesses. This study was carried out to evaluate antioxidant capacity of the methanol, chloroform and ethyl acetate extracts of V. Amygdalina and as antiproliferative agents in inhibiting the growth of human colon adenocarcinoma cell line (HT-29) and human breast adenocarcinoma cell line (MCF-7). It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (2.69 mg/g GAE in concentration 0.5 mg/mL); more effective than methanol and chloroform extract. This fraction also exhibited fairly good antioxidant activity with in both TBA (17.39% mg/g GAE) and FTC (12.65% mg/g GAE) methods. Antiproliferative study indicated that V. Amygdalina crude extract have great potential as antiproliferative compounds against various cancer cell lines and can significantly inhibit the growth of MCF-7 and HT-29 cancer cells. The methanol V. Amygdalina extract has a broad spectrum of activity by inhibiting the growth of MCF-7 and HT-29 cancer cells.

Keywords: Vernoniaamygdalina, antioxidant activity, anti-proliferative activity, MCF- 7 and HT-29 cancer cells.