Abstract

OPTIMIZE OF SOME BIOREACTOR PHYSICAL CONDITIONS AFFECTING ON ACCUMULATION OF ACTIVE COMPOUNDS IN SUSPENSION CULTURES OF EGYPTIAN DATE PALM (SAMANY CULTIVAR).

*Taha H.S, A.M.Abd El-Kawy and M.K. El-Bahr

Abstract

Date palms (Phoenix dactylifera L., Arecaceae) are one of oldest cultivated plants in the Middle East and North Africa, and used in folk medicine for treatment of various diseases. The concept of this study is investigate the effects of agitation rates (100 to 160 rpm) and aeration (40 to 80 % dissolved oxygen DO) as a stirred tank bioreactor (STB) physical conditions on mass cells proliferation, total phenolic (TP) and total flavonoid (TF) contents. Further, evaluate the antioxidant activity in suspension cultures of Egyptian date palm (Samany cultivar). The highest value of mass cell proliferation recorded with the agitation rate of 120 rpm and 40 (%) of DO. However, the highest values of TP and TF contents 6.45 mg gallic acid equivalents (GAE) per 100 g fresh cells, and 1.12 mg/100g rutin equivalents, respectively recorded with 120 rpm and 60 % of DO. Whereas the antioxidant activities of methanolic extracts were evaluated in vitro using scavenging assays of 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical. Effectiveness scavenging concentration (IC50) on DPPH radical (2.45 mg/l) estimated with 120 rpm and 80 (%) of DO. These results indicated that date palm (Samany cultivar) cell cultures could be physical controlled in bioreactor, further considered a good natural source for active and medicinal compounds production.

Keywords: Date palm, suspension, bioreactor, flavonoids, total phenolic.