Abstract

ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF 20(29)-LUPEN-3-OL FROM THE ROOT EXTRACT OF DACRYODES EDULIS AND ITS ANTIMICROBIAL POTENCIES AGAINST SOME CLINICAL AND PLANT PATHOGENS

*Nna P. J., Edori O. S. and David-Sarogoro N.

Abstract

Roots of Dacryodes edulis are used in traditional medicine as remedy to stomach ailment, parasitic skin diseases, dysentery and yellow fever. This study was aimed at isolation and characterization of pure compound that enhances the medicinal potency of the plant part by means of spectroscopic methods which include Thin Layer Chromatography, FT-IR, nuclear magnetic resonance spectroscopy (1H-NMR, 13C-NMR and 2D) as well as the antimicrobial activity of the isolated compound against some clinical and plant pathogens. 20(29)-lupen-3-ol was isolated from the plant and its antimicrobial activity was examined on 6 bacteria and 11 fungi and also 4 standard control drugs were used in the study. The antimicrobial screening of the isolated compound showed that the compound has higher potential for medicinal values. The compound exhibited high activity against some of the microbes used. It was sensitive to Aspergillus flavus, Aspergillus fumigates, Coniophora puteana, fibrophoria vaillentii, fasarium proliforatum, Rhizopus sp, Serpula lacryneus with zones of inhibition 32, 30, 27, 28, 29, 30, 28mm respectively. The antibacterial screening shows that the compound was sensitive against Methicillin resist staph aureus, staphylococcus aureus, Escherichia coli and Salmonella typhi with zones of inhibition 30, 27, 28 and 27mm respectively. The results accounts for the trado-medical claims of the plant and thus could be used as a candidate of drug in curing some diseases caused by the microorganisms examined in the study.

Keywords: Aspergillus flavus, Aspergillus fumigates, Coniophora puteana.