CYTOTOXIC AND IMMUNOMODULATION EFFECT OF ROASTED FLAXSEED AND SOYBEAN EXTRACTS
Dharshini, T.Thirunalasundari, S.Sumayaa
Abstract
Research is focusing on the search for new types of natural
chemotherapeutic agents derived from plant sources which are
providing to be excellent sources of new compounds. Thus, Soybean
(Glycine max (L)) and flaxseed (Linuim usitatissimum L.) are known
to have a number of health benefits ranging from the well documented
many chronic diseases. The present research article was aimed to
study the cell viability and cytotoxic activity of roasted form of
soybean and flaxseed extracts. The in-vitro methods used for the
determination of cell viability by trypan blue dye exclusion method
and cytotoxicity by MTT and SRB assay against MCF-7. The cell
viability also showed significant result.The IC50 value of roasted form
of soybean and flaxseed are 125μg & 80μg, 135 μg & 140μg
respectively in MTT and SRB assay whereas this extracts were found to be non-toxic to
normal PBMC. It is proved that the extracts of roasted soybean and flaxseed exhibits
significant anticancer potential non-toxicity on MCF-7 cell line.
Keywords: soybean, flaxseed, MTT, SRB, PBMC and MCF-7 cell line.
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