Abstract

EVALUATION OF EXPRESSION VECTORS DESIGNS FOR EXPRESSION OF RECOMBINANT MONOCLONAL ANTIBODY IN CHO CELLS

*Sharvari Joshi, Dr. Kiran Mangaonkar and Dr. Archana Krishnan

Abstract

Monoclonal antibodies are emerging and most promising class of recombinant proteins. The significant value of monoclonal antibodies is due to their high efficiency in the treatment of wide range of variety of diseases. The complex nature of the monoclonal antibody poses a great challenge for the upstream and downstream process. Proportionate expression of heavy and light chain gene and post translational modification assembly of the molecule are the major factors in the efficient expression of monoclonal antibody. Different types of genetic optimizations are required for efficient expression of monoclonal antibody. The design of an expression vector has proven to be effective in increasing antibody expression and quality. The cumulative objective of the research work is to evaluate different vector designs for the expression of monoclonal antibody. The efficiency of different mammalian expression vector is explored for the expression of recombinant IgG1 antibody using Chinese Hamster Ovary (CHO) cells. The antibody expression was analyzed in stable pools followed by analysis of single cell clones and fed batch expression. The noticeable amount of protein expression was observed in two expression vectors, in-house developed vector showed proficient expression and highest productivity in CHO cells. Conclusion: The vector designing is significant factor for effective production of whole monoclonal antibody in CHO cells.

Keywords: Monoclonal antibody, mammalian expression, expression vector, Chinese hamster ovary (CHO).