Abstract

VALIDATED UPLC/Q-TOF-MS METHOD FOR STRUCTURAL IDENTIFICATION AND QUANTITATIVE DETERMINATION OF ANTIHYPERTENSIVE DRUGS

Hamid Khan*

Abstract

In the presented work the ultra-performance liquid chromatographic/quadrupole time-of-flight mass spectrometric (UPLC/Q-TOF-MS) method has been developed for Structural Identification and Quantitative Determination of Antihypertensive Drugs such as Losartan, Telmisartan, Valsartan Candesartan and Irbesartan,. For identification of drugs, the Q-TOF mass spectrometer was operated in negative ionization mode and quantification was done using the MS/MS transitions at m/z 421.2 to 127.00 for Losartan, m/z 513.18 to 469.13 for telmisartan, m/z 435.02 to 157.00 for valsartan, m/z 439.1 to 309.9 for Candesartan and m/z 427.2 to 193.08 for Irbesartan. The chromatographic separation was achieved on Acquity UPLCTM BEH C18 (100.0 × 2.1 mm, 1.7μm) column using isocratic mobile phase consisting of acetonitrile-2mM ammonium acetate (50:50, v/v) at a flow rate of 0.25 mL/min. The calibration curves were linear over the concentration range of 1-1000 ng/mL for all the drugs. The developed method was validated according to ICH guidelines. The method is helpful in Structural Identification and Quantitative Determination of Antihypertensive Drugs.

Keywords: UPLC/Q-TOF-MS, Antihypertensive Drugs, Structural Identification.