Abstract

EXTRACTION PURIFICATION AND CHARACTERIZATION OF CELLULASE OF CELLULASE ENZYME COMPLEX FROM TRICHODERMA T5

Ansari Nayab Taher*

Abstract

The cellulase enzyme complex was extracted from Trichoderma T5. The enzyme was purified using column chromatography. The effect of different parameters on the activity of enzyme was determined, it was found that the enzyme had optimum temperature of 30° C, the optimum pH was 5.5, the optimum substrate concentration was found to be 2.0% W/V of carboxyl methyl cellulose also it was found that time of incubation influences the enzyme activity it was found that the optimum time of incubation was 96 hours after which the activity decreases. Thus, the cellulase enzyme complex was extracted from Trichoderma T5 and further purified by column chromatography and it proves to be a efficient method of purification of enzyme complex and the activity of enzyme is effected by different parameters.

Keywords: Purification, Cellulase enzyme, Trichoderma T5.