Abstract

ESTIMATION OF BETULINIC ACID IN EXTRACTS AND FORMULATIONS CONTAINING ALBIZIA LEBBECK BY HPLC

Pratima Tatke*, Sonal Desai1, S. Y. Gabhe 

Abstract

A triterpene acid, betulinic acid has been studied extensively because of its wide range of biological properties specially its anti-tumor activity. The present method discusses a new, simple and sensitive reversed phase HPLC-DAD method for quantitative estimation of betulinic acid in stem bark extract and formulations of Albizia lebbeck. Betulinic acid was analysed using MZ-Analytical C18 column (250 mm X 4.6 mm, 5 μm) as stationary phase. Elution was carried out using acetonitrile: water (92:08, v/v) at flow rate of 1.0 ml/min. Detection was carried at 205 nm. Column oven temperature and injection volume were kept at 25 0C and 20 μl, respectively. Linearity of the proposed method was found to be over the range 0.3-60 μg/ml. LOD and LOQ were found to be 0.1 μg/ml and 0.3 μg/ml, respectively. The method was found to be accurate with % recovery of 92.40 - 96.54 %.

Keywords: Albizia lebbeck; Betulinic acid; Fabaceae; Shirish; Standardization.