Abstract

ISOLATION AND IDENTIFICATION OF BACILLUS THURINGIENSIS BY BIOCHEMICAL METHOD

Dr. Prasanna Purohit*

Abstract

Bacillus thuringiensis is a gram positive rod shaped, aerobic spore forming soil bacterium, which produces crystalline insecticidal proteins within the cytoplasm at the time of sporulation. These insecticidal crystal proteins are highly toxic to various insects belong to Lepidopteron Coleopterus and Dipteran families. Isolation and characterization to obtain efficient Lepidopteran specific Bacillus thuringiensis as bio-control agent from different soil samples. Out of 50 soil samples collected, only 20 samples were used for the isolation. Sodium acetate selection method was used and the results were positive for presence of Bacillus thuringiensis bacteria. More than one method or biochemical test is used for isolate Bacillus thuringiensis strains from different soil samples. The isolates which are positive for crystal protein production were invariable endospore formers but the morphology of the crystal protein inside. These isolated Bacillus thuringiensis can be used in future for the transformation techniques as a biopesticide and may be on the control of Teak defoliator.

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