Abstract

PHYTOCHEMICAL STUDIES ON THE STEROLS OF METHANOLIC LEAF EXTRACTS OF MEDICINALLY IMPORTANT PLANT AILANTHUS EXCELSA (ROXB.) USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY

Mudit Kumar* and Sudhir Singh Gangwar

Abstract

Objective: The present study was conducted to identify the sterols from methanol extract of medicinally and economically useful leaves of Ailanthus exc elsa (Roxb.) using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (S witzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: HPTLC finger printing of sterols of methanol extract of leaves revealed at Rf 0.59 maroon colour hence sterols are detected. Conclusions: With the results of HPTLC analysis and above Rf value we have concluded the presence of sterols in the extract.

Keywords: Ailanthus excelsa (Roxb.) leaves, sterols, HPTLC Fingerprinting.