Abstract

ASSESSMENT OF GENETIC DIVERSITY IN WATERMELON (CITRULLUSLANATUS (THUNB.) BY USING SDS-PAGETHROUGH GENETIC ANALYSIS SOFTWARE

Rajesh Goud Gajula*, Dr. Shailima RD Vardhini, Dr. Sujatha Edupuganti, G.Raghavendar

Abstract

Seed storage Protein profiles of five varieties of CitrullusLanatus wereanalysed by sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE). Total seed storage protein of CitrullusLanatus were resolved on 10% SDS Polyarylamide gels showedvariations in their banding pattern. The dendrogram constructed byNTSys PC software. The SAHN clustering based on UPGMAalgorithms has put the all accession into different cluster groups. TheJaccord’s coefficient also calculated. Maharaja Variety and Superdragon verity has close genetic relation. Shahanshah and Maharajahave highest variation among five cultivars.Jaccard’s similarity showsthat Maharaja and Shahanshah are 55% similar which is less similarity in all cultivars,Maharaja and Super Dragon are 73% similar, Maharaja and Durgesh has 73% similarity,Super Dragon and Durgesh are 71% similar. More work is needed to identify the specificgenes in the cultivars, quantitative trait loci (QTL) works are carrying out to identify andcorrelate the genes for different traits using RAPD Technique.

Keywords: Citrullus Lanatus, SDS-PAGE, Storage protein, Genetic diversity, Electrophoresis, NTSys PC, SAHN, UPUGMA, Jaccord’s Coefficient.