Abstract

FINGERPRINTING OF THE FLAVONOIDS FROM AILANTHUS EXCELSA (ROXB.) LEAVES USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY

Dr. Ravindra C. Sutar*

Abstract

Objective: The present study was conducted to identify the Flavonoids from methanol extract of medicinally and economically useful leaves of Ailanthus excelsa (Roxb.) using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: HPTLC finger printing of Flavonoids of methanol extract of leaves revealed Different Rf showing fluorescence hence flavonoids are detected. Conclusions: With the results of HPTLC analysis and Rf values Flavonoids have been concluded in the extract.

Keywords: Ailanthus excelsa (Roxb.) leaves, Flavonoids, HPTLC Fingerprinting.