Abstract

VALIDATION METHODS OF AMLODIPINE DRUG WITH COMPARITIVE STUDY OF STANDARD AND SAMPLE BY USING UV-SPECTROSOCPY

Kollaboina Sekhar*, Abbas S. Md, Chandra Mohan G., Eranna P., Hamana Naik D., Kalyan P., Mahesh T., Rajesh J. and Varun B.

Abstract

The present study of this article is validation methods of amlodipine with comparative studies of crude form by using UV-spectroscopy. During the validation process the drug will studied with comparatively. The absorbance ranges from 336 – 338nm. UV-spectroscopy is the most effective method for validation studies for comparison purpose. The process which is used in the validation method of these article will obey the Beer-Lambert‟s law. The method was validated in terms of Accuracy, Precision, Linearity, Ruggedness, Robustness, LOD, LOQ and Assay. Results of analysis were validated statistically and by recovery studies. From that it was observed that there was no interference of impurities or excipients during the validation of drug in formulation. Further these methods utilise no additional chemical reagents for development of any colouring species with amlodipine as observed in some studies in literature and hence this method is very accurate, simple and les time consuming.

Keywords: Amlodipine; Validation; UV-spectroscopy; Comparative studies; Tablet; Analysis.