Abstract

EVALUATION OF ANTIDIABETIC AND ANTIOXIDATIVE EFFECT OF SEED OF EUJENIA JAMBOLANA IN STREPTOZOTOCIN INDUCED DIABETIC MALE RAT

Kishalay Jana, Abhinandan Ghosh, Tushar Kanti Bera, Debidas Ghosh*

Abstract

Present study focus on the effect of ethyl acetate fraction of seed ofEujenia jambolana (E. jambolana) in streptozotocin (STZ) induceddiabetic male rats. Rats were divided into four groups (n=6), control,diabetic, ethyl acetate fraction treated (200 mg/kg body weight/day for35 days) and glibenclamide treated (20 mg/kg body weight/day for 35days) group. Fasting blood glucose levels (FBG) were measured of allanimals. Important carbohydrate metabolic enzymes i.e. hexokinase,glucose-6-phosphatase and glucose-6-phosphate dehydrogenase weremeasured in hepatic tissue. Serum insulin, glycated hemoglobin(HbA1C) and glycogen content also measure in hepatic and skeletalmuscle tissue. For antioxidant activity we measured catalase (CAT),peroxidase (Px) superoxide dismutase (SOD) along with thequantification of thiobarbituric acid reactive substances (TBARS) and conjugated diene (CD)in hepatic and renal tissue. Histological study of pancreatic tissue also evaluated. Serumglutamate oxaloacetate transaminase (GOT) and serum glutamate pyruvate transaminase(GPT) levels were measured for toxicity assessment. The FBG levels, serum insulin, HbA1C,three carbohydrate metabolic enzymes activity and antioxidant enzymes along with lipidperoxidation end product were recovered significantly after fraction treatment in compare tountreated diabetic group. Degeneration of pancreatic islets cell were noted in untreateddiabetic group, but after fraction treatment it was recovered towards the control animals. Allresults were compared with glibenclamide treated group, a standard antidiabetic drug. Thisstudy indicates that the ethyl acetate fraction of seed of E. jambolana is effective for thetreatment of diabetes mellitus without any toxicity.

Keywords: Antidiabetic, Antioxidant, E. jambolana, Oxidative stress