Abstract

IS ESSENTIAL OIL OF LIBYAN ARTEMISIA HERBA ALBA PLANT EFFECTIVE AGAINST MULTI-DRUG RESISTANT PSEUDOMONAS AEROGINOSA BACTERIA

Salwa Ibraheim Eltawaty*, Salema R. M. Qowaider, Sanaa Osman Yagoub and Asma Alramli

Abstract

Due to the noticeable increasing rates of resistance of pathogenic bacteria in general and Ps. aeruginosa in specific towards common used antibiotics and due to the promising biological activities expected from medicinal plants, this in vitro study aimed to investigate the antibacterial performance of essential oil of Libyan Artemisia herba alba plant against standard and clinical multi-drug resistant Ps.aeruginosa comparing with six antibiotics references. Methods: Disc diffusion assay and agar dilution method were used for evaluation of antibacterial performance and MIC, respectively. Result: Both standard and clinical Ps. aeruginosa were actively inhibited by the essential oil. 6.25mg/ml is the MIC revealed from tested oil against standard Ps. aeruginosa ATCC 27853. High significant differences shown in tested oil and antibiotics effects (Pv ≤0.01). No effect shown from Augmentin and Ceftriaxone, moderate growth inhibition shown from Ceftazidim and active growth inhibition performance shown from Ciprofloxacin, Gentamicin and the tested essential oil of plant. Conclusion: This study conclude that the tested essential oil of Artemisia herba alba wild grown in north east of Libya has a powerful antibacterial activity against both sensitive and multi-drug resistant Ps.aeruginosa and suggests that this oil could be considered as a promising source for new antibacterial pharmaceutical preparations.

Keywords: Multi-drug resistant Ps.aeruginosa, essential oil, Artemisia herba alba, MIC. Corresponding autor: Dr. Salwa I. Eltawaty.