Abstract

SPECTROPHOTOMETRIC DETERMINATION OF DRUGS AND PHARMACEUTICALS BY USING FOLIN-CIOCALTEU REAGENT

Naveen Reddy Pailla and Bhaskar Kuthati*

Abstract

A simple, fast, selective and accurate spectrophotometric method has been outlined for the assessment of five phenolic drugs namely Balsalazide, Desvenlafaxine, Formoterol, Nelfinavir mesylate, Xipamide. This method is based on the development of blue coloured chromogen due to reduction of tungstate and/ or molybdate in Folin- Ciocalteu reagent by Drug in alkaline medium. The coloured species has an absorption maximum at 770nm, 755nm, 735nm,760nm, 740nm and the Beer’s law obeys over the concentration range of 7–48μg/mL, 2-14 μg/mL, 5-35 μg/mL, 3.5-24.5 μg/mL, 11-77 μg/mL respectively for the drugs Balsalazide, Desvenlafaxine, Formoterol, Nelfinavir mesylate, Xipamide. This method has been validated according to ICH guidelines and applied to the analysis of pharmaceuticals.

Keywords: Spectrophotometry, Folin-Ciocalteu reagent, drugs, pharmaceutical formulations.