Abstract

SYNTHESIS OF dl-1-(4-HYDROXYBENZYL)-1,2,3,4-TETRAHYDRO-β- CARBOLINE AND EVALUATION OF ITS BIOLOGICAL ACTIVITY

Nazmul Qais* and Nusrat Jahan

Abstract

Bischler-Napieralsky reaction was utilized to synthesize novel tetrahydro-β-carboline compound namely, dl-1-(4-hydroxybenzyl)- 1,2,3,4-tetrahydro-β-carboline, where POCl3 in combination with ZnCl2 was used as condensation reagent and the synthesized compound was evaluated for antioxidant activity and analgesic activity. Anti-oxidant study of the synthesized compound was investigated by DPPH assay method and the IC50 value of the compound was 48.42 g/ml compared to the standard ascorbic acid, IC50 value was 2.83 μg/ml. The peripheral analgesic activity of the compound was investigated by acetic acid induced writhing method in mice at the dose of 60 mg/kg and 80 mg/kg body weight. This study showed significant analgesic activity having writhing inhibition of 86.78% and 88.43% respectively compared to the standard aceclofenac at a dose of 50 mg/kg body weight and 64.46% inhibition of writhing.

Keywords: THBCs, Bischler-Napieralsky reaction, DPPH assay, Inhibitory Concentration (IC50), Acetic acid-induced writhing method, Analgesic activity.